High performance phytosynthesized nanostructures as biosensor platforms for detecting pathogenic microorganism from food matrices

NanoBIOPAT

PN-III-P2-2.1-PED-2021-0042

(652PED/2022)

Recent progresses in the nanotechnology field revealed the uses of nanomaterials for developing control systems to ensure food quality and safety.

Project goal

The project goal is represented by the development of a technology for the phytosynthesis of metallic nanomaterials, using extracts from cruciferous crops waste (cabbage, broccoli, turnip, radish, etc.) with application as a sensing platform for the detection of microbial pathogens from food matrices (meat, fruits, dairy products).

Contracting authority: The Executive Unit for the Financing of Higher Education, Research, Development and Innovation (UEFISCDI)

Implementation period: 28/06/2022-20/06/2024

Budget: 274.447 lei

Project responsible: Dr. Habil. Radu Claudiu Fierăscu

  • Consortium
  • General objectives
  • Phase I
  • Phase II
  • Phase III
  • Results dissemination
Consortium

Coordinator:

University POLITEHNICA of Bucharest,

Project director: Dr. Ing. Camelia Ungureanu

Partner:

National Institute for Research & Development in Chemistry and Petrochemistry – ICECHIM Bucharest

Project responsible: Dr. Habil. Radu Claudiu Fierăscu


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General objectives

Applying this method to obtain nanostructures with plants waste highlights the importance of minimising and re-using residues from primary and secondary processing via chemical and social intervention, to contribute to the sustainability needs of the planet and its inhabitants. Also, will be enhanced agro food quality through performant and eco-friendly detecting systems for different pathogens which alter food matrixes and transform them more rapidly into food wastes.

Phase I

Evaluation of the possibilities of obtaining phytosynthesized nanostructures for sensors application.

Implementation period: 28/06/2022 – 31/12/2022

Activities of the partner ICECHIM:

  • Developing protocols for obtaining natural extracts with enhanced concentrations of phytochemicals;
  • Obtaining and characterizing natural extracts from a phytochemical point of view;
  • Results dissemination
Phase II

Development of technology for obtaining phytosynthesized nanostructures with control of properties and morphology.

Implementation period: 01/01/2023 – 31/12/2023

Activities of the partner ICECHIM:

  • Synthesis and characterization of phytosynthesized nanomaterials;
  • Optimization of working parameters for nanostructure synthesis, intermediate activity;
  • Results dissemination
Phase III

Optimization of working parameters for nanostructure synthesis – final stage. Demonstration and validation of the proposed technology

Implementation period: 01/01/2024 – 20/06/2024

Activities of the partner ICECHIM:

  • Optimization of working parameters for nanostructure synthesis, final activity;
  • Evaluation of results at laboratory level – improving process performance;
  • Development of technical documentation for the technology for obtaining the sensor platform.
  • Results dissemination
Results dissemination

Published papers

  1. Ungureanu, C.; Tihan, G.T.; Zgârian, R.G.; Fierascu, I.; Baroi, A.M.; Răileanu, S.; Fierăscu, R.C. Metallic and Metal Oxides Nanoparticles for Sensing Food Pathogens—An Overview of Recent Findings and Future Prospects. Materials 2022, 15, 5374. https://doi.org/10.3390/ma15155374
  2. Diana Elena Vizitiu; Daniela Ionela Sardarescu; Irina Fierascu; Radu Claudiu Fierascu; Liliana Cristina Soare; Camelia Ungureanu; Elena Cocuta Buciumeanu; Ionela Catalina Guta; Letitia Maria Pandelea, Grapevine plants management using natural extracts and phytosynthesized silver nanoparticles; Materials 2022, 15(22), 8188; https://doi.org/10.3390/ma15228188
  3. Fierascu, I.C.; Fierascu, I.; Baroi, A.M.; Ungureanu, C.; Spinu, S.; Avramescu, S.M.; Somoghi, R.; Fierascu, R.C.; Dinu-Parvu, C.E. Phytosynthesis of Silver Nanoparticles Using Leonurus cardiaca L. Extracts. Materials 2023, 16, 3472. https://doi.org/10.3390/ma16093472
  4. Baroi, A.M.; Fierascu, R.C.; Matei, R.I.; Fistos, T.; Fierascu, I.; Ungureanu, C. Cruciferous Waste Material from Valuable Resource for Nanoparticle-Based Sensors. Proceedings 2023, 90, 3. https://doi.org/10.3390/proceedings2023090003
  5. Buică, G.; Ungureanu, C.; Baroi, A.M.; Hosu, I.S.; Fierascu, I.; Fierascu, R.C. Colorimetric Detection of Bacteria Using Au Nanoparticles. Proceedings 2023, 90, 12. https://doi.org/10.3390/proceedings2023090012

Scientific meetings

  1. IDEA (IDEA, Novelty, Invention and craft) International Exhibition, Abony, Hungary – High performance phytosynthesized nanostructures as biosensor platforms for detecting pathogenic microorganism from food matrices, Camelia Ungureanu, Radu Claudiu Fierascu, George Octavian Buica, Irina Fierascu, Cristian Valeriu Pirvu, Roxana Ioana Matei (Gold medal)
  2. International Exhibition of Inventions and Innovations “TRAIAN VUIA – 2022 Timisoara – High performance phytosynthesized nanostructures as biosensor platforms for detecting pathogenic microorganism from food matrices, Camelia Ungureanu, Radu Claudiu Fierascu, George Octavian Buica, Irina Fierascu, Cristian Valeriu Pirvu, Roxana Ioana Matei (Gold medal)
  3. 7th International Inventions Fair – ISIF’22 – High performance phytosynthesized nanostructures as biosensor platforms for detecting pathogenic microorganism from food matrices, Camelia Ungureanu, Radu Claudiu Fierascu, George Octavian Buica, Irina Fierascu, Cristian Valeriu Pirvu, Roxana Ioana Matei (Gold medal)
  4. 22nd Romanian International Conference on Chemistry and Chemical Engineering (RICCCE), Sinaia, 7-9 September 2022 (RICCCE 22), The development of phytosynthesized silver nanoparticles mediated by Raphanus sativus L. extracts, Camelia Ungureanu, Diana Gabriela Hutu, Irina Fierascu, Ana Maria Baroi, Raluca Somoghi, Radu Claudiu Fierascu.
  5. “PRIORITIES OF CHEMISTRY FOR A SUSTAINABLE DEVELOPMENT”, PRIOCHEM XVIII, ICECHIM – Bucharest, 26-28 October 2022, Synthesis of gold nanoparticles using watercress seeds Camelia Ungureanu, Petre Miron, Diana Gabriela Hutu, Anda Maria Baroi, Raluca Somoghi, Irina Fierascu, Radu Fierascu.
  6. International Scientific Symposium CURRENT TRENDS IN NATURAL SCIENCES 2023, ‘Green’ synthesis of metallic nanoparticles: applications for biosensor platform, Camelia Ungureanu, Anda Maria Baroi, Ioana Silvia Hosu, Irina Fierascu, Zgârian Roxana, Tihan Grațiela, Radu Claudiu Fierascu
  7. Cruciferous waste material from valuable resource for nanoparticle-based sensors, Anda Maria Baroi, Radu Claudiu Fierascu, Anda Maria Baroi, Roxana Ioana Matei (Brazdis), Toma Fistos, Irina Fierascu, Camelia Ungureanu, “Priorities of Chemistry for a Sustainable Development” PRIOCHEM XIXth Edition, 11-13 Octombrei 2023, Bucuresti
  8. the 19th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 11-13 Oct-2023 PRIOCHEM, Colorimetric Detection of Foodborne Pathogens Using Au-Nanoparticles, George Buică, Camelia Ungureanu, Anda Maria Baroi, Ioana Silvia Hosu, Irina Fierascu, Radu Claudiu Fierascu
  9. International Warsaw Invention Show “IWIS” 12-14 December 2023, Method of detection of Escherichia coli bacteria through electrochemically coupled surface plasmon resonance using electrodes modified with phytosynthesized gold nanoparticles and procedure for their preparation, Camelia Ungureanu, Alexandra Constantinescu, Cristian Valeriu Pîrvu, Radu Claudiu Fierăscu, Irina Fierăscu, Anda-Maria Baroi, Roxana Ioana Brazdis (Matei), Toma Fistoș, Irina Elena Chican
  10. Kaohsiung International Invention and Design EXPO, 30 Noiembrie-02 Decembrie 2023, Method of detection of Escherichia coli bacteria through electrochemically coupled surface plasmon resonance using electrodes modified with phytosynthesized gold nanoparticles and procedure for their preparation, Camelia Ungureanu, Alexandra Constantinescu, Cristian Valeriu Pîrvu, Radu Claudiu Fierăscu, Irina Fierăscu, Anda-Maria Baroi, Roxana Ioana Brazdis (Matei), Toma Fistoș, Irina Elena Chican
  11. International Scientific Symposium Current Trends in Natural Sciences, May 16-18, 2024, Colorimetric Detection of Staphylococcus aureus using Metallic Nanoparticles. George BUICĂ, Camelia UNGUREANU, Anda Maria BAROI, Ioana Silvia HOSU, Irina FIERASCU, Radu Claudiu FIERASCU
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Book chapters

  1. Fierascu, I., Baroi, A.M., Fistos, T., Brazdis, R.I., Sardarescu (Toma), I.D., Fierascu, R.C. (2022). Bryophytes and the Nanotechnology: Recent Developments and Perspectives. In: Murthy, H.N. (eds) Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-97415-2_29-1
  2. Role and application of nanosensors in crop protection for disease identification, Baroi, A.M., Ungureanu, C., Calinescu, M.F., Vizitiu D., Sardarescu I.D., Ortan A., Fierascu, R.C., Fierascu, I., Nanoformulations for Sustainable Agriculture and Environmental Risk Mitigation, 2023, pp. 118–141

Workshop

  1. Phytosynthesis – synthesis of the future. The bioeconomy approach. within “Priorities of Chemistry for a Sustainable Development” PRIOCHEM XIXth Edition, 11-13 Octombrei 2023, Bucuresti, over 20 participants, Book of Abstracts (pag 59), ISSN 2601 – 419X and ISSN-L 2601 – 4181 – English ed., CD-ROM, other Electronic Supports.
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Patent application

  1. Method of detection of Escherichia coli bacteria through electrochemically coupled surface plasmon resonance using electrodes modified with phytosynthesized gold nanoparticles and procedure for their preparation, Camelia Ungureanu, Alexandra Constantinescu, Cristian Valeriu Pîrvu, Radu Claudiu Fierăscu, Irina Fierăscu, Anda-Maria Baroi, Roxana Ioana Brazdis (Matei), Toma Fistoș, Irina Elena Chican, patent application A00549/2023