Development of plant extracts and innovative phytosynthesized nanostructured mixtures with phytotherapeutic applications in order to reduce biocenotic stress in horticultural crops

BIOHORTINOV - Component project 3

6PCCDI/2018 - PC3

Project goal

In recent decades, there has been a high interest in the scientific field of nanotechnology to obtain and characterize nanometric architectures obtained by "green chemistry" methods, in order to reduce or eliminate the use and generation of substances dangerous to human health and the environment. Nanoparticles with different compositions, shapes, sizes and controlled polydispersity were developed and synthesized. Regardless of the techniques for obtaining the nanoparticles, toxic substances, organic solvents or toxic reducing agents are used. As an alternative, the phytosynthesis of nanomaterials is eco-friendly, easy to scale and less expensive. From this perspective, the project proposes an absolutely innovative and original approach for obtaining extracts and phytosynthesized nanometric architectures (mono and bimetallic nanoparticles) with practical applications in a field of special importance at national level: combating the main fungal diseases affecting vine and apple culture (two of the national cultures of special importance).

Contracting authority: Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI)

Implementation period: 01/03/2018-30/06/2021

Total budget: 1.291.000 lei

Budget for the partner ICECHIM: 443.000 lei

Component project director: Dr. Chim. Radu Claudiu Fierăscu

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  • Partners
  • General objective
  • Specific objectives
  • Phase I
  • Phase II
  • Phase III
  • Phase IV
  • Patents
General objective


The project proposes an original approach for obtaining extracts and phytosynthesized nanometric architectures with practical applications in combating the main fungal diseases that affect vine and apple cultures.

Elements of originality:

  • use of alternative plants, belonging to the spontaneous flora (pteridophytes);
  • the use of phytosynthesized nanomaterials to combat fungal diseases of the target crops (at the level of real crops);
Specific objectives


  • obtaining and characterizing natural extracts obtained from spontaneous flora (pteridophytes);
  • phytosynthesis and characterization of metal nanoparticles;
  • the study of the synergistic action of the proposed solutions, both at the in vitro level and on the target cultures;
  • dissemination of results and protection of intellectual property rights
Phase I

Development of modern strategies and protocols for obtaining plant extracts and nanostructured mixtures for target applications.

ICECHIM activities:

  • Selection of the most promising plant materials, for obtaining bio-active extracts and for the phytosynthesis of bio-active nanostructures;
  • Development of laboratory protocols for obtaining and analytical characterization of natural extracts and nanostructured mixtures;
Phase II

Obtaining and characterizing plant extracts and nanostructured mixtures; evaluation of the effect at laboratory level.

ICECHIM activities:

  • Optimization of extraction methods in order to obtain superior extraction yields; Obtaining nanostructured mixtures
  • Characterization of nanostructured mixtures by physico-chemical methods (spectroscopic, microscopic, methods based on the interaction of ionizing radiation with matter);
Phase III

Large-scale experimentation of proposed solutions.

ICECHIM activities:

  • Scaling up the proposed model for obtaining active natural extracts.
  • Experimenting the model proposed in Stage II. Verification of the effectiveness of plant extracts and nanostructured mixtures in protected areas and storage. Their expansion in the orchard;
  • Experimenting the model proposed in Stage II. Verification of the effectiveness of plant extracts and nanostructured mixtures in protected areas. Their expansion in the vineyard.
Phase IV

Development of technological sequences for the proposed solutions.

ICECHIM activities:

  • Contributions to the development of technological sequences and technical documentation on the control of plant fungi and nanostructured mixtures of the main fungal diseases affecting apple and vine cultivation, pre- and post-harvest.
Patents
  • Irina Fierăscu, Radu Claudiu Fierăscu, Toma Fistoș, Cristina Liliana Soare, Camelia Ungureanu, Diana Vizitiu, Oana Alexandra Drăghiceanu, Alina Păunescu, Ecological antifungal composition to control phytopathogenic strains affecting vines and method of obtaining it, Patent application A00158/2019;
  • Cristina Liliana Soare, Irina Fierăscu, Radu Claudiu Fierăscu, Camelia Ungureanu, Mirela Florina Călinescu, Mihaela Dobrescu, Anca Nicoleta Șuțan, Ecological antifungal solution to control phytopathogenic strains affecting apple crops and method of obtaining it, Patent application A00159/2019;
  • Irina Fierăscu, Radu Claudiu Fierăscu, Toma Fistoș, Cristina Liliana Soare, Camelia Ungureanu, Diana Vizitiu, Natural fungicidal composition for controlling grapevine manna and the method of obtaining it, Patent application A00073/2020;