Through nanotechnology towards the next generation dental restorative materials

NANODENT

PN-III-P4-PCE-2021-0292

(92PCE/2022)

The solution developed in the research project will lead to a completion of the teeth treatment through the possibility of having antibacterial properties and slow release after the cavity is closed, with enhanced mechanical properties.

Project goal

The main goal of the project is to propose a potential dental restorative material with simultaneous antibacterial properties and enhanced mechanical strength, based on substituted apatitic nanomaterials (decorated with metallic nanoparticles) and natural compounds, applicable in cement base (glass-ionomer cements).

Contracting authority: Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI)

Implementation period: 09/05/2022-31/12/2024

Budget: 1.200.000 lei

Project director: Dr. Habil. Radu Claudiu Fierăscu

  • Scientific concept
  • Novelty
  • Objectives
  • Phase I
  • Phase II
  • Phase III
  • Results 
Scientific concept

The scientific concept of the project is based on some specific logical steps:

S1. Synthesis of modified apatitic nanomaterials (MAN), substituted with different metals such as zinc, silver, titanium etc., and further decorated with mono or bimetallic phytosynthesized nanoparticles and metallic oxides nanoparticles (such as silver and silver oxide nanoparticles, zinc and zinc oxides nanoparticles, silver/zinc bimetallic nanoparticles, etc.), with controlled morphologies and properties;

S2. Demonstration of their enhanced properties;

S3. Enriching the MAN with natural compounds (commercially available polyphenols, such as eugenol, gallic acid, or catechin, for example) – NNC;

S4. Obtaining nanoparticle-based platforms (incorporation of apatitic materials enhanced with natural compounds in glass-ionomer cements – NGI);

S5. Demonstration of the enhanced properties of the obtained

nanoparticle-based platforms (NGI);

S6. Demonstration of their efficiency in the laboratory model (simulated conditions);

S7. Validation of the proposed solution.


Novelty

The main goal of the project is to propose a potential dental restorative material with simultaneous antibacterial properties and enhanced mechanical strength, based on substituted apatitic nanomaterials (decorated with metallic nanoparticles) and natural compounds, applicable in cement base (glass-ionomer cements). The degree of novelty of the proposed project emerges from the properties of the proposed material related with potential final application: simultaneous antibacterial and enhanced mechanical strength of the developed nanomaterials-based structures, applicable in the cement base (glass-ionomer cements). 

Objectives

The specific objectives that will ensure the achievement of project aim are:

SO1. development of substituted apatitic materials with enhanced antimicrobial properties;

SO2. phytosynthesis of mono and bimetallic nanoparticles based on “green chemistry” approaches (with extracts obtained from autochthonous plants belonging to the Lamiaceae family – Thymus serpyllum L., 1753, Satureja hortensis L., 1753, Melissa officinalis L., Mentha spicata L., etc.) and their application for the decoration of the apatitic materials, in order to obtain modified apatitic nanomaterials (MAN) with enhanced antimicrobial properties

SO3. enrichment of the MAN with natural compounds (NNC)

SO4.incorporation of NNC in glass-ionomer cements and the demonstration of their efficiency

Phase I

Development of apatitic nanostructures with potential use in improving current dental restorative materials.

Deployment period: 09/05/2022 – 31/12/2022

Activities:

  • Synthesis and characterization of apatitic materials
  • Assessing the possibilities of incorporating modified apatitic nanomaterials (MAN) into glass ionomer cements
  • Assessment of antimicrobial properties and biocompatibility of apatitic nanomaterials – Part I
  • Synthesis and characterization of phytosynthesized metallic and bimetallic nanoparticles
  • Evaluation of the antimicrobial properties of phytosynthesized NPs
  • Dissemination of results and project;
Phase II

Optimizing the decoration of apatitic materials. Enrichment of apatitic materials modified with natural compounds – part I.

Deployment period: 01/01/2023 – 31/12/2023

Activities:

  • Assessment of antimicrobial properties and biocompatibility of apatitic nanomaterials – Part II
  • Evaluation of the mechanical properties of new apatitic materials
  • Optimization of apatitic materials taking into account their morphologies, antimicrobial, mechanical properties and biocompatibility
  • Optimization of phytosynthesys parameters and decoration of apathetic nanomaterials for the development of modified apatitic nanomaterials (MAN).
  • Optimizing the decoration of apatitic materials with phytosynthesized nanoparticles to achieve optimal properties
  • Obtaining and characterizing MANs enriched with natural compounds
  • Dissemination of results and project
Phase III

Enrichment of apatitic materials modified with natural compounds – part II. Incorporation of modified apatitic materials enhanced with natural compounds in glass ionomer cements (for obtaining platforms based on nanomaterials – NGI) and study of their influence on the mechanical properties of GIC. Demonstration and validation of the improved properties of the obtained NGIs.

Deployment period: 01/01/2024 – 31/12/2024

Activities:

  • Evaluation of antimicrobial effects and biocompatibility of MAN enriched with natural compounds
  • Evaluation of the mechanical properties of apatitic materials enriched with natural compounds
  • Obtaining and characterizing glass ionomer cements based on nanomaterials enriched with natural compounds (NGI) with improved mechanical properties.
  • Evaluation of antimicrobial properties and NGI biocompatibility
  • Evaluation of the release of active compounds under conditions similar to real ones (synthetic saliva solutions).
  • Evaluation of antibacterial effects and biocompatibility under conditions similar to real ones (synthetic saliva solutions)
  • Evaluation of mechanical properties, adhesion and thermal insulation properties in conditions similar to real ones (synthetic saliva solutions); validation of the proposed solution
  • Development of technical documentation for technology and products and patent applications
  • Dissemination of results and project
Results 
Expected results
  • at least 8 published articles in Q1/Q2 scientific journals, preferably open-access publications
  • at least 10 participations at international conference
  • at least 6 participations at inventions exhibitions,
  • publication of a book on the project topic at a foreign publication house;
  • workshop
  • at least two patent applications
Obtained results
ISI articles
Scientific conferences
Invention exhibitions
Book
Workshop
Patent applications
Phase reports