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National Institute for Research & Development in Chemistry and Petrochemistry

ICECHIM

G6: Biotechnology & bioanalysis

About us

The 6th Research Group carries out research activities in the field of Biotechnology & Bioanalysis, the research directions being: 1. Biotechnologies for environmental protection and remediation  Biodegradation of waste and pollutants  Biotechnologies to remedy contamination 2. Agri-food and industrial biotechnologies for obtaining biologically active products  Microbial technologies for obtaining substances with antimicrobial properties  Pharmaceutical biotechnologies  Biotechnologies for obtaining products with uses in the light chemical and food industry (new sources of enzymes) 3. Bioanalysis methods for quality control of products and biotechnological processes in the field of health, environment and agri-food  Advanced methods and techniques of analysis, control and quality assurance of biotechnological substances and products  Development of sensors, biosensors and immunosensors for the detection of toxic compounds, pollutants and metabolites in the environment and agri-food products. Within the 6th reasearch group, there is a Collection of Microorganisms that contains fungi, yeasts and bacteria, which allows and supports the biotechnological approach of our research activities. Microorganisms have the ability to adapt to a variety of environmental conditions, their versatility making them useful for various biotechnological applications, such as removing pollutants from the environment, bioremediation or transformation into harmless compounds such as carbon dioxide and water. Enriching the collection of microorganisms by isolating them from various sources and maintaining them are of permanent concerns of microbiologists. The 6th research group is a multidisciplinary team, consisting in 2 chemists, 2 biochemists, 2 biologists, 1 engineer and 1 technician (3 CSI, 2 CSII, 2 CSIII; 6 doctors in science). The specialists our group actively participate in:  Evaluation of scientific research projects carried out at national (UEFISCDI) and European level (INTAS experts)  Review activity as references of specialized magazines from Romania and abroad - Roum. Biotechnol. Letters, Biosensors & Bioelectronics (Elsevier), Food Biotechnology, Sensors, Toxins, Biomachine, Biosensors, Bioelectrochemistry, Biomedical and Environmental Science, etc.  Supporting the activity of the Romanian Society of Bioengineering and Biotechnology (SRBB) (www.srbb.ro), an organization affiliated since 1993 to the European Biotechnology Federation  Didactic activities carried out in higher education institutions (University of Bucharest, Polytechnic University of Bucharest, etc.). Through Capacity project 49/CP/I/2007, the laboratories of the Biotechnology & Bioanalysis team were arranged according to GLP norms as follows:  Microbiology Laboratory (respecting the Rules of Good Laboratory Practice-RBPL regarding the flow of personnel, materials and samples)  Laboratory for (BIO) Sensors and optical methods of bioanalysis  Laboratory for Separation Methods

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Team
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Senior Scientist 1st degree, Leader of Biotechnology & Bioanalysis Research Group

Dr. chem.

Extension: 144

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Senior Scientist 1st degree, Deputy Leader of Biotechnology & Bioanalysis Research Group

Dr. chem.

Extension: 170

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Senior Scientist 1st degree, General Manager of INCDCP-ICECHIM

Dr. biochem.

Extension: 102

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Senior Scientist 1st degree,

Dr. biol.

Extension: 110

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Senior Scientist 1st degree

Dr. Sc. HDR

Extension: 171

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Senior Scientist 3rd degree

Dr. chem.

Extension: 144

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Senior Scientist 3rd degree

Dr. biol.

Extension: 170

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Senior Scientist 3rd degree

Dr. biol.

Extension: 170

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Senior Scientist 3rd degree

Dr. chim.

Extension: 144

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Senior Scientist 3rd degree

Dr. Eng.

Extension: 110

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Scientific Researcher (CS)

Biologist

Extension: 144

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Research Assistant

Eng.

Extension: 110

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Eng.

Eng.

Extension: 110

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Technician

Techn.

Extension: 144

Research equipment
International projects
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Innovative MXene-peptides for electrochemical miniaturized platforms dedicated to tumor biomarker detection

The goal of MXEPEPMIN is to develop miniaturized biosensors based on innovative MXene-peptide nanomaterials for the detection of lung cancer biomarkers, CEA and EGFR.

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Proiect 33/2017 MANUNET „Noi sisteme analitice pentru evaluarea prezentei toxinelor in industria lactatelor” (acronim TOX-HAZ-ASSESS)

Proiect 33/2017 MANUNET „Noi sisteme analitice pentru evaluarea prezentei toxinelor in industria lactatelor” (acronim TOX-HAZ-ASSESS)

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INNOVATIVE FULLERENOL - HYDROGELS BASED NANOMATERIALS FOR HEALTH DIAGNOSTIC AND CARE APPLICATIONS - FULSENS-GEL

The main objective of the project consists in the development of an innovative nanomaterial, based on combination of the elastic, flexible and resistant hydrogels with functional nanomaterials based on fullerenols

National projects
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ENHANCING FOOD SAFETY THROUGH DRUG RESIDUES MONITORING IN FOOD CHAIN USING INNOVATIVE BIOSENSITIVE PORTABLE TOOLS PN-IV-P7-7.1-PED-2024-1277

The main goal of the project is to develop miniaturized, cost-effective and robust biosensing instruments based on an innovative MXene-based nanomaterial customized with specific bioreceptors – an emerging class of 2D- conductive materials with super

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BIOGENIC AMINES DETECTION IN FOOD BASED ON AN INNOVATIVE OPTO-ELECTROSENSITIVE PLATFORM

Scopul principal al proiectului îl constituie dezvoltarea unor platforme opto-electrosensibile, miniaturizate, rentabile și robuste bazate pe un nanomaterial inovator pentru determinarea specifică și sensibilă a aminelor biogene (BAs) din alimente

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BioConstrMater

Biotechnological strategies for innovative construction materials incorporating bacterial bioproduct

Results
  • Publications
  • Books
  • Conferences
  • Patents
  • Other
Publications
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Cristina Firincă, Lucian-Gabriel Zamfir, Mariana Constantin, Iuliana Răut, Maria-Luiza Jecu, Mihaela Doni, Ana-Maria Gurban, and Tatiana Eugenia Sesan

Innovative Approaches and Evolving Strategies in Heavy Metal Bioremediation: Current Limitations and Future Opportunities

Journal of Xenobiotics

https://doi.org/10.3390/jox15030063

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Răut, I.; Constantin, M.; Suică-Bunghez, R.; Firincă, C.; Alexandrescu, E.; Gîfu, I.C.; Doni, M.; Zamfir, L. G.; Gurban, A.M.; Jecu, L.

Extracellular Biosynthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Synthesized by Filamentous Fungi

J. Fungi

https://doi.org/10.3390/jof10110798

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Stefaniu A.; Neagu, G.; Albulescu A.; Radu, N.; Pirvu, L.C

In silico comparison of quantum and bioactivity parameters of a series of natural diphenyl acetone analogues; in vitro Caco-2 studies on three main chalcone derivates

Symmetry, 3222087, 2024

https://doi.org/10.3390/sym16101383

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Idu, A.-A.; Albu Kaya, M.G.; Rău, I.; Radu, N.; Dinu-Pîrvu, C.-E.; Ghica, M.V.

Novel Collagen Membrane Formulations with Irinotecan or Minocycline for Potential Application in Brain Cancer

Materials 2024, 17, 3510.

https://doi.org/10.3390/ma17143510

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Bostan, I.S.; Mihaila, M.; Roman, V.; Radu, N.; Neagu, M.T.; Bostan, M.; Mehedintu, C

Landscape of Endometrial Cancer: Molecular Mechanisms, Biomarkers, and Target Therapy

Cancers 2024, 16, 2027

https://doi.org/10.3390/cancers16112027

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C Firincă, LG Zamfir, M Constantin, I Răut, L Capră, Diana Popa, ML Jinga, AM Baroi, RC Fierăscu, NO Corneli, C Postolache, M Doni, AM Gurban, Luiza Jecu, TE Șesan,

Microbial Removal of Heavy Metals from Contaminated Environments Using Metal-Resistant Indigenous Strains,

J. Xenobiot

https://doi.org/10.3390/jox14010004

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Viviana Roman, Mirela Mihaila, Nicoleta Radu, Stefania Marineata, Carmen Cristina Diaconu and Marinela Bostan

Cell Culture Model Evolution and Its Impact on Improving Therapy Efficiency in Lung Cancer

Cancers 2023, 15, 4996

https://doi.org/10.3390/ cancers15204996

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Alexandru Ciric, Nicoleta Radu, Marilena Gabriela Olteanu Zaharie, Georgeta Neagu, Lucia Camelia Pirvu , Mihaela Begea and Amalia Stefaniu

Potential Antitumor Effect of Functional Yogurts Formulated with Prebiotics from Cereals and a Consortium of Probiotic Bacteria

https://doi.org/10.3390/foods12061250

https://doi.org/10.3390/foods12061250

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Mariana Constantin, Iuliana Răut, Raluca Suica-Bunghez , Cristina Firinca, Nicoleta Radu , Ana-Maria Gurban , Silviu Preda , Elvira Alexandrescu , Mihaela Doni and Luiza Jecu

Ganoderma lucidum-Mediated Green Synthesis of Silver Nanoparticles with Antimicrobial Activity

Materials 2023, 16, 4261

https://doi.org/ 10.3390/ma16124261

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Ana-Maria Gurban , Lucian-Gabriel Zamfir , Petru Epure, Ioana-Raluca S, uică-Bunghez , Raluca Mădălina Senin, Maria-Luiza Jecu, Maria Lorena Jinga and Mihaela Doni

Flexible Miniaturized Electrochemical Sensors Based on Multiwalled Carbon Nanotube-Chitosan Nanomaterial for Determination of Nitrite in Soil Solutions

Chemosensors 2023, 11(4), 224;

https://doi.org/10.3390/chemosensors11040224

Books
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Calin M; Raut I.; Gurban A.-M.; Doni M.; Radu N.; Jecu L.

Archaeometrical study of selected objectives from Golesti Museum of Viticulture and Pomiculture

Chapter5: Microbial contamination of wooden cultural heritage artifacts of selected objectives from Golesti Museum

ISBN978-619-91466-1-3

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Radu N.; Babeanu N.; Cornea P.; Calin M.; Jecu L.; Gurban A.M; Doni, M;

Archaeometrical study of selected objectives from Golesti Museum of Viticulture and Pomiculture

Chapter 4:Paintings characteristics from inside of wooden church achieved in the period 1750-1850

ISBN 978-619-91466-1-3

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M Calin, I Raut, ML Arsene, M Doni, AM Gurban, L Jecu

Practical exemples regarding the valorization of cultural heritage through archaeometry studies using nuclear techniques and other scientific methods

Chapter 4. Isolation and identification of microorganisms involved in the biodeterioration of cultural heritage artifacts

ISBN: 978-973-0-27114-0

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I Raut, M Calin, ML Arsene, M Doni, AM Gurban, L Jecu

Valorization of cultural heritage through archaeometry studies using nuclear techniques and other scientific methods. General considerations and short literature survey

Chapter 5. Microbial deterioration of textile and wood artifacts

ISBN: 978-973-0-27114-0

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F. Oancea, I. Raut, T.E .Sesan, M. Doni, M. Popescu, V. Zamfiropol-Cristea, L. Jecu

Trichoderma spp. - applications in agriculture and horticulture

Chapter 8 - Formulation of the Multifunctional Trichoderma Strains

ISBN 978-606-16-0900-0

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F., Oancea, I. Raut, T.E. Sesan, M. Doni, M. Popescu, V. Zamfiropol-Cristea, L. Jecu

Trichoderma spp. - applications in agriculture and horticulture

Chapter 7. Trichoderma Strains as Plant Biostimulants in High Residues Farming Systems

ISBN 978-606-16-0900-0

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Răut I., Şesan T.E., Arsene M.L.*, Badea-Doni M., Jecu L., Călin M., Vasilescu G., Oancea F.

Trichoderma spp. - applications in agriculture and horticulture

Chapter 5. Volatile and non-volatile metabolites of Trichoderma with biocontrol implications

ISBN 978-606-16-0900-0

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Arsene M.L.*, Jecu L., Badea-Doni M., Raut I., Oancea F., Calin M

Trichoderma spp. - applications in agriculture and horticulture

Chapter 4. Bioactivity and biological role of secondary metabolites produced by Trichoderma spp

ISBN 978-606-16-0900-0

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Arsene M.L., Badea-Doni M., Jecu L*, Răut I., Călin M

Trichoderma spp. - applications in agriculture and horticulture

Chapter 3. Chemical structure of secondary metabolites

ISBN 978-606-16-0900-0

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A.F. Danet, M. Badea – Doni

Flow Injection Analysis of Food Additives

Chapter 32: Determination of Lipid Oxidation by Chemiluminescence Reagents - pp. 623-638, CRC Press, Francis & Taylor Group, Editors: Claudia Ruiz-Capillas, Leo M. L. Nollet (2015)

ISBN 9781482218190

Conferences
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C. Firincă, I. Răut., M.-L. Jinga, L. Capră, R. Constantinescu, A.-M. Gurban, L.-G. Zamfir M. Doni, M.L. Jecu, M. Constantin, C. Postolache, T. E. Șesan

Bioremediation strategy for chromium contaminated soils

Next-Chem Tehnologii Inovatoare Trans-Sectoriale – workshop exploratoriu, ediția a V-a, 22-23 May 2023, Bucharest, Romania

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R. Ianchiş, E. Alexandrescu, I.C. Gîfu, I.C. Mihăescu, C.L. Nistor, C. Petcu, L.-G. Zamfir, M. Doni, A.-M. Gurban

Novel biopolymer-polyaniline composites designed for sensing applications

4th International Conference on Emerging Technologies in Materials Engineering – EmergeMAT, 4–5 November 2021, Bucharest, Romania

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L.-G. Zamfir, P. Epure, C. Mitrea, M. Constantin, I. Raut, M.-L. Jecu, M. Doni, A.-M. Gurban

A comparative study of different nanomaterials used in the development of sensitive electrochemical sensors for nitrite determination and monitoring in soil

6th edition of International Conference on Analytical and Nanoanalytical Methods for Biomedical and Environmental Sciences "IC-ANMBES 2022", June 8–10 2022, Brașov, Romania

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Marilena Gabriela Zaharie, Mihai Caramihai, Montserrat Adroguer, Paqui Blánquez, Tom Kuppens and Nicoleta Radu

Virtual Reality and Mixed Reality Education: A Solution for Today’s Education in a Circular Bioeconomy

16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020.

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Mariana Călin, Iuliana Răut, Ana-Maria Gurban, Mihaela Doni, Nicoleta Radu, Gelu Vasilescu and Luiza Jecu

Assessment of Biological Contamination from Wooden Artifacts of Golesti Museum

16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020

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Iuliana RĂUT, Mariana CĂLIN, Ana-Maria GURBAN, Luiza CAPRĂ, Mihaela DONI, Nicoleta RADU, Gelu VASILESCU and Luiza JECU

A Biotechnological Process for Valorizing Poultry Feathers through Keratinolytic Cladosporium Isolates

16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020.

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Naomi Tritean, Mariana Călin, Iuliana Răut, Ana-Maria Gălan, Alexandru Vlaicu, Diana Constantinescu-Aruxandei and Florin Oancea

Extraction of Proteins from Microalgae Using Lytic Enzymes Produced by Trichoderma Strains

16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020.

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Nicoleta Radu, Narcisa Babeanu, Petruta Cornea, Mariana Calin , Luiza Jecu, Iuliana Raut, Ana Maria Gurban and Mihaela Doni

Painting Degradation from Inside Wooden Churches Achieved in the Period 1750–1850

16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020.

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Indira Galit, Nicoleta Radu, Ana Aurelia Chirvase , Mihai Dan Caramihai , Montserrat Sarrà, Paqui Blánquez, Tom Kuppens , Charlotte Adriaensens and Narcisa Babeanu

Developments of Tertiary Level Studies in Biotechnologies and Their Applications in Environmental Bioengineering

16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020

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Ana-Maria Gurban , Petru Epure, Iuliana Răut, Mariana Călin, Nicoleta Radu, Claudia Ninciuleanu, Bogdan Trică, Luiza Jecu, Elvira Alexandrescu and Mihaela Doni

Different Approaches in Designing Sensitive Tools Based on Nanocomposite Materials for Biological Analytes Detection

16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020.

Patents
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Sandu, T, Sarbu A, Jecu L, Iordache T, Gavrilsa AM, Apostol L, Radu AL, Zaharia AM, Arsene ML

Perle polimerice cu continut de particule magnetice si procedeu de obtinere a acestora

Brevet RO 134595/2023

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Cornea PC, Oancea F, Voaideş CM, Răut I, Boiu Sicuia OA, Constantinescu Aruxandei D, Doni M, Jecu L

Consorţiu microbian multifuncţional pentru solubilizarea fitosiliciului şi procedeu pentru utilizarea acestuia

Brevet RO133468B1/2023.

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Oancea F, Călin M, Constantinescu D, Răut I, Doni M, Arsene ML, Jecu L,

Compozitie de biostimulant pentru plante pe baza de lana, si procedeu de obtinere,

Brevet RO133240 B1/2021.

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V Fruth, Todan L, Predoana L, Poenaru A, Ariciov L, AR Leonte, Ciobanu EM, Petcu G, RM Ion, L Iancu, LM Jecu, I Raut, M Calin

Procedeu de obtinere a unor películe nanocompozite destinate protejarii componentelor arhitecturale litice ale patrimoniului cultural

Brevet RO134390/2021.

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Oancea, F. Constantinescu D, I Răut, M Doni, O S Dima, ML Arsene, ML Jecu

Procedeu de solubilizare a siliciului din material vegetal

Brevetul RO132515/ 2021

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Oancea Florin, Velea Sanda, Popescu Mariana, Răut Iuliana

Process for conditioning biostimulating microorganisms for plants, in the form of an effervescent tablet

131927/30.08.2019

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F Oancea, I Raut, TE Sesan, M Doni, M Popescu, L Jecu

Procedure for selecting consortia of microorganisms for the treatment of plant material

131224B1/2019

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F Oancea, I. Raut, Abraham Beata, Szabolcs Lanyi Stefan, M Doni, L Jecu

High yield process for the selection of consortia of microorganisms with action to stimulate cultivated plants

131225

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Oancea F, Sesan T E, M Doni, Raut I, Vlăsceanu G A, Negru G. L Jecu

Silicium-based biostimulating composition for foliar application

3/2/30.01.2019

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F Oancea, I Raut, TE Sesan, M Doni, M Popescu, L Jecu

Procedure for selecting consortia of microorganisms that release and solubilize biosilicium from plant material

131828B1/2019

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Other
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Doni M; Jecu L.; Gurban AM.; Radu N.; Constantin M; Raut I; Firinca C.; Zamfir L

CONFERENCE-AWARDS

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R.M. Ion; N. Ion; L. Iancu; N. Radu

Silver Medal awarded to Patent request to EUROINVENT, 11th Edition, European Exhibition of Creativity and Innovation, May 18, 2019, Iasi, Romania.

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R.M. Ion, N. Ion, L.Iancu, N. Radu

Gold Medal awarded to Patent request 17th edition of PROINVENT, March 20-22, 2019, Cluj Napoca, Romania