G6: Biotechnology & bioanalysis
The 6th Research Group carries out research activities in the field of Biotechnology & Bioanalysis, the research directions being: 1. Biotechnologies for environmental protection and remediation Biodegradation of waste and pollutants Biotechnologies to remedy contamination 2. Agri-food and industrial biotechnologies for obtaining biologically active products Microbial technologies for obtaining substances with antimicrobial properties Pharmaceutical biotechnologies Biotechnologies for obtaining products with uses in the light chemical and food industry (new sources of enzymes) 3. Bioanalysis methods for quality control of products and biotechnological processes in the field of health, environment and agri-food Advanced methods and techniques of analysis, control and quality assurance of biotechnological substances and products Development of sensors, biosensors and immunosensors for the detection of toxic compounds, pollutants and metabolites in the environment and agri-food products. Within the 6th reasearch group, there is a Collection of Microorganisms that contains fungi, yeasts and bacteria, which allows and supports the biotechnological approach of our research activities. Microorganisms have the ability to adapt to a variety of environmental conditions, their versatility making them useful for various biotechnological applications, such as removing pollutants from the environment, bioremediation or transformation into harmless compounds such as carbon dioxide and water. Enriching the collection of microorganisms by isolating them from various sources and maintaining them are of permanent concerns of microbiologists. The 6th research group is a multidisciplinary team, consisting in 2 chemists, 2 biochemists, 2 biologists, 1 engineer and 1 technician (3 CSI, 2 CSII, 2 CSIII; 6 doctors in science). The specialists our group actively participate in: Evaluation of scientific research projects carried out at national (UEFISCDI) and European level (INTAS experts) Review activity as references of specialized magazines from Romania and abroad – Roum. Biotechnol. Letters, Biosensors & Bioelectronics (Elsevier), Food Biotechnology, Sensors, Toxins, Biomachine, Biosensors, Bioelectrochemistry, Biomedical and Environmental Science, etc. Supporting the activity of the Romanian Society of Bioengineering and Biotechnology (SRBB) (www.srbb.ro), an organization affiliated since 1993 to the European Biotechnology Federation Didactic activities carried out in higher education institutions (University of Bucharest, Polytechnic University of Bucharest, etc.). Through Capacity project 49/CP/I/2007, the laboratories of the Biotechnology & Bioanalysis team were arranged according to GLP norms as follows: Microbiology Laboratory (respecting the Rules of Good Laboratory Practice-RBPL regarding the flow of personnel, materials and samples) Laboratory for (BIO) Sensors and optical methods of bioanalysis Laboratory for Separation Methods
Senior Scientist 1st degree, General Manager of INCDCP-ICECHIM
Senior Scientist 1st degree, Leader of Biotechnology & Bioanalysis Research Group
Senior Scientist 2nd degree, Deputy leader of Biotechnology & Bioanalysis Research Group
PalmSens Compact Electrochemical Interface
The PalmSens instrument is a battery-powered and handheld instrument which allows the application of most of the relevant voltammetric and amperometric techniques. The PSTrace program for Windows provides the requirements for using PalmSens as electrochemical interface by means of a desktop PC or (mini-) notebook. The instrument can be connected by means of a USB or serial (RS-232) cable or by means of wireless Bluetooth.
Hettich Universal 32R Refrigeration Centrifuge
The Hettich Universal 32R Refrigeration Centrifuge Model 1610-01 runs perfectly at high and low speeds, is versatile and has a small footprint. Features include: accommodates tubes up to 100mL; microlitre tubes up to a max RCF of 23,90; short run-up and run-down times; efficient cooling within -20 to +40C; metal housing; stainless steel chamber; lid locking and lid dropping protection.
aCOLyte fully automated (SuperCount) colony counter-SYNBIOSIS
aCOLyte is a fully automated (SuperCount) colony counter, which enables to count colonies on spread, pour or spiral plates and instantly store the images and results in a professional report format with the time and date recorded for GLP/GMP compliance.
Sunrise Absorbance microplate reader – Tecan Life Science
Tecan’s Sunrise absorbance microplate reader is an ideal solution for a range of applications, such as ELISAs, enzyme kinetics, cell viability studies, veterinary assays and many more, offering all the functionality needed, including advanced 12-channel optics for fast, multichannel absorbance reading of ELISAs, a temperature control function for enzyme kinetic assays, and a tunable wavelength function for wavelength scanning. It supports a broad wavelength range – from 340 to 750 nm – and can measure an entire 96-well plate in less than six seconds, ensuring it is ready to meet the needs of routine diagnostic and research labs.
Autolab PGSTAT101 Potentiostat/Galvanostat Metrohm
The Autolab/PGSTAT101 is an affordable potentiostat/galvanostat and in combination with the powerful NOVA software, can be used for most of the standard electrochemical techniques. The small footprint of PGSTAT101 allows to place this high quality potentiostat/ galvanostat on a crowded workbench. Analog and digital inputs and outputs for interfacing and controlling Autolab accessories and external devices are available. The Autolab PGSTAT101 comes with an internal dummy cell and is 9 x 21 x 15 cm3 for 2.1 kg.
VCX 750 – Ultrasonic Processors for Small and Medium Volume Applications
VCX 750 Watt Ultrasonic Processors is used to safely process a wide range of organic and inorganic materials, from 250 microliters to 1 liter. Typical applications include sample preparation, dispersion, cell lysing, desegregation, homogenization, particle size reduction, soil testing, transesterification (including biodiesel production), nanotechnology (including nanoparticle and Graphene dispersion), acceleration of chemical reactions, degassing and atomization.
Spectrofluorometer FP-6500 Jasco
The FP-6500 is the research-grade spectrofluorometer with a minimum 1 nm resolution and a highest sensitivity S/N ratio 200 or greater for the Raman band of water. The FP6500 is especially designed to meet requirements in biochemistry as kinetics, stopped-flow, titration, or anisotropic measurements, as well as to meet the GLP/GMP requirements. Built-in low-pressure Mercury lamp and UV cut filter enable to compliance the GLP/GMP with validation software which is available as standard.
Moisture determination balance WPS30-Partner
Moisture Determination Balance is a rapid and accurate method for moisture content and dry weight analysis of a wide range of products and materials. It automatically and simultaneously dries and weighs solid samples for the determination of moisture content. It provides a continuous direct readout for both weight and percentage moisture loss through the entire cycle, providing moisture content data as a percentage of the initial mass or the residual dry mass.
Thermo Scientific NanoDrop-OneC, UV-Vis Spectrophotometer
The NanoDrop OneC is a Stand-Alone instrument with local control and Built-in touch screen, for improved measurement accuracy and contaminant identification. Using NanoDrop OneC spectrophotometer it is possible to quantify and qualify DNA, RNA, and protein samples by using only 1-2 µL, in seconds. The full-spectral data is obtained before decide to use samples in downstream applications. With contaminant analysis, sample information alerts and on-demand technical support, the NanoDrop One is the ideal UV-Vis spectrophotometer for life science research.
µStat EC – DropSens portable BiPotentiostat/Galvanostat
µStat ECL is a portable BiPotentiostat/Galvanostat combined with a specific Electrochemiluminescence Cell that performs electrochemiluminescence studies with DropSens Screen-Printed Electrodes (SPEs). A BiPotentiostat/Galvanostat (± 4 V DC potential range, ± 40 mA maximum measurable current) and a Si-Photodiode integrated in the ECL Cell (Spectral response range: 340 – 1100nm) are combined and offer a simple, compact and portable solution in the field of ELECTROCHEMILUMINESCENCE to be used with SPEs. StatECL is controlled by DropView 8400 Software.
Mixer Mill MM400 RETSCH
The mixer mill MM 400 is a compact versatile bench-top unit, which has been developed specially for dry, wet and cryogenic grinding of small amounts of sample. It can mix and homogenize powders and suspensions in only a few seconds. It is also perfectly suitable for the disruption of biological cells as well as for DNA/RNA and protein extraction. With its high performance and great flexibility the mixer mill MM 400 is a unique product in the market. The fields of applications consist in agriculture, biology, chemistry / plastics, construction materials, engineering / electronics, environment / recycling, food, geology / metallurgy, glass / ceramics, medicine / pharmaceuticals.
ELMASONIC E30 Ultrasonic Cleaner Bath
ELMASONIC ultrasonic bath is an economic cleaning for all purposes, offering top cleaning results with Sweep technology, using high ultrasonic power with the latest processor-controlled generator technology, being durable and economic. Cleaning by ultrasound is intensive, gentle to the material and environment friendly all at the same time. Tiny micrometric vacuum bubbles are created which implode very fast: this process is called cavitation. It produces highly energetic jets which remove efficiently all dirt particles from any item immersed in the cleaning bath. The ceramic heating elements in the Elmasonic E units assist the cleaning process.
Heidolph Rotary Evaporator, Laborota 4000WB
Heidolph rotary evaporator meet the requirements of any laboratory, being designed for simple and complex distillations of volumes from 50 mL to 3L. Economic modular design allows for a choice of 5 interchangeable condenser assemblies with or without plastic safety coating, water or oil bath and manual or motor lift. Long lasting graphite-filled PTFE vacuum seal between drive and vapor tube provides leak-free, greaseless operation. Rotary drive is electronically controlled, range from 20 to 270 rpm. Adjustable gliding water and oil baths have encapsulated heating elements to prevent scalding and feature digital bath temperature display.
Thermo Spectronic BioMate UV-Vis spectrophotometer
Thermo Spectronic BioMate Double Beam UV-Vis Spectrophotometer with Brand quartz Cuvette, with soft for enzymatic and microbial kinetics for rapid, reliable nucleic acid and protein analysis, represents a grating-based spectrophotometer that can also be used as a general purpose instrument to measure other life science applications such as kinetics or multi wavelength analysis.
Gallenkamp heating and drying oven is used for general production and research in various branches. It is economical and environmentally friendly, thanks to its enhanced thermal insulation offering optimum thermal separation of the inner and outer casing, having a high degree’s of safety. The interior chamber is made from stainless steel coated with aluminium (CLAD). Fitted with fixed 6 shelf runners and 4 removable chrome plated wire grid shelves. The top vent is fitted with a clip to hold a mercury-in-glass thermometer.
Biochemical oxygen consumption determination system – OxiTop IS
BOD/aerobic degradation represents a respirometric measuring system for aerobic measuring, also for BOD self check measurements, 6 measuring units. Stirring platform IS 6. OxiTop®-IDS wireless measuring heads with Bluetooth® LE technology for operation with Multi 3620 IDS or Multi 3630 IDS, memory for 360 measuring points, with backlit graphic LCD and control LED, for routine BOD also operable without IDS multiparameter instrument.
portable Mettler Toledo FiveGo F2-Meter pH-meter
FiveGo2 pH-Meter is an ergonomic, robust and intuitive portable meter which offers a quality solution for mobile applications in the laboratory, at-line and even outdoors. It gets quality measurements of pH, conductivity, ORP or dissolved oxygen, having a large, well-structured display with intuitive button arrangement and simple menu.
FINNIGAN Surveyor Plus HPLC, Thermo Scientific, USA
The Thermo Scientific Surveyor Plus HPLC is a robust modular system with a suite of integrated features to increase application flexibility and efficiency, being specifically optimized for the most demanding HPLC applications and allowing full customization for our laboratory’s applications. The versatility of the modular design, featuring custom quaternary pumping solutions, provides flexibility for a wide variety of applications, from routine compound analysis to more complex matrices, offering short cycle times, high sample capacity and low flow rate performance.
BIOLOG MicroStation-taxonomic identification of microbial strains
BIOLOG represents a semi-automated platform for microbial identification and community analysis in pharmaceutical, industrial, agricultural and academic research and development. It provides powerful cellular analysis used in many applications, including microbial identification and characterization, as well as advanced phenotypic analysis for cells of all types, from microbial to mammalian. Biolog’s Software supporting FDA 21 CFR Part 11 has a wide range of products for the identification, detection and characterization of bacteria, yeast, filamentous fungi, and microbial communities.
MINIPLUS 3 Peristaltic Pump Gilson
The MINIPULS® 3 is a compact high-performance, low-pulse, peristaltic pump with interchangeable pump heads for delivering a diverse variety of liquids—from biological to chemical—without altering the critical components within the liquid. Six different channel and flow rate configurations are available. Utilizing positive displacement pump technology, liquid flows nearly pulse-free as a result of as many as ten stainless steel rollers. The MINIPULS 3 produces smoother flows with excellent accuracy and precision, even at high flow rates. It has an adjustable speed stepper motor and a high-quality precision bore tubing which assure reproducible flow rates.
KLF 2000 BIOREACTOR
KLF is an in situ serializable bench-top bioreactor made of glass, being impressive due to its ease of operation and the many combinations and possibilities of extension. Equipped for batch, fed-batch or perfusion processes, with an accurate process control with high-grade probes, the KLF bioreactor is controlled with BioSCADA, a flexible software that is adaptable to user requirements. This bioreactor provides best in class oxygen transfer rates due to powerful agitator, allowing a full sterilization in place with stirred media enables fast sterilization of media with minimal negative impact to media components.
Oakton pH/Ion 2100 Benchtop pH/mV/Ion/°C/°F Meter with pH Electrode
The pH 2100– bench meter measures pH, millivolts, relative millivolts and temperature. The pH 2100 meter also measures ion concentration. This meter are equipped with a large customized LCD (Liquid Crystal Display) with simultaneous display of the measured values for easy reading. It is most ideal for routine pH/Ion Concentration measurement in indoor applications.
OLYMPUS BX51 Microscope
Olympus BX51 microscope is configured for DIC, phase contrast, and bright field applications, with the possibility to choose between a binocular, ergo binocular, or trinocular viewing head. BX51 microscope allows reflected light observation as well as transmitted light observation. Reflected light mode offers dark field (where the angle of sample illumination is 90°) and bright field (where the sample is illuminated sideways) observation. The microscope is equipped with a color digital camera with a resolution of 5 megapixels and can be connected to a powerful computer system. It is equipped with the U-DICT prism with U-ANT analyzer. The system as a whole is designed to provide accurate color reproduction of the observed sample.
SYSTEC VX/VE Series Autoclave
The Systec VX/VE Series autoclaves are designed for use in laboratories, for sterilization in steam or a steam-air mixture. Systec autoclaves can be used in all laboratory applications, even in demanding sterilization processes: the sterilization of liquids (such as nutrient and culture media), solids (such as instruments, pipettes, glassware), waste (destructive sterilization of liquid waste in bottles, or solid waste in destruction bags) and biological hazards in safety laboratories. The process procedures are simpler, safer and more precise.
FASTER BSC Microbiological Protection Hood
Faster SafeFAST Classic vertical laminar flow cabinets are Class II Microbiological Safety Cabinets – designed and built to performance requirements of the EN-12469:2000 European Standard and NSF/ANSI 49 American Standard, with 70% of the air re-circulated via the main H14 HEPA/ULPA filter according to EN 1822 within the cabinet, whilst the remaining 30% is discharged through an exhaust H14 HEPA/ULPA filter. SafeFAST Classic is used for product, personnel and environmental protection while handling harmful agents pathogenic to human beings and/or animals, in a wide range of applications such as: Microbiology, Virology, Hematology, Cell culture, Genetics, Handling of hazardous agents to human beings or animals.
ECO Chemie/Metrohm µAutolab III Galvanostat/Potentiostat Electrochemical Tester
The µAUTOLAB Type III in combination with GPES 4.9 software can be used for all standard DC-electrochemical techniques. This instrument has a built-in analog integrator, analog and digital inputs and analog and digital outputs, making it a versatile instrument despite its non-modular nature. The analog input allows to measure an external signal (e.g. voltage) and the analog output can be used to control µAutolab accessories or external devices (e.g. Rotating Disk Electrode). The digital inputs and outputs can be used to control devices such as mercury drop electrodes.
DLAB D1008 Micro/Mini Centrifuge
DLAB mini-centrifuge is ideal for microfiltration and quick spin downs from the walls and caps of microcentrifuge tubes. The compact design takes up less than six inches of bench space. The D1008 offers quick and convenient spinning, the operation being easy and functioning by simply closing the lid for acceleration and opening the lid for deceleration, or alternatively use the on/off switch to control.
BAS EPSILON 5 ELECTROCHEMICAL DETECTOR
BAS Epsilon 5 electrochemical detector has an electrochemical flow cell, an (optional) injection valve, and a CC-5 Compartment. Electrochemical cells are very sensitive to stray electrical currents. For that reason, the CC-5 Compartment serves as a Faraday cage to shield the electrochemical cell from such currents. Several options allow the optimization of the system to the specific application.
UNIMAX1000 -Heidolph Incubators
UNIMAX1000 incubator represents a unique modular system which allow mixing, shaking and tempering – all without the need for an additional steaming cabinet. Fast heat up, no condensation develops in the system, is ideal for large-volume of high vessels, such as Erlenmeyer flasks up to 1000 ml. This incubator possess foldable hood, an electronic temperature control with two-digit display of setpoint and actual value, and an electric convection heater with fan.
BINDER – Cooling incubator KB
BINDER cooling incubators for microorganisms operate within a temperature range of between -5 and 100 °C delivering maximum precision even at high ambient temperatures and combining energy efficiency with high performance and usability. With its extensive programming options and homogenous incubation conditions even when fully loaded, this cooling incubator covers a wide range of applications. BINDER’s incubator it can be used with flexibility, all while delivering results that are accurate and reproducible. A compressor cooling unit takes care of cooling in the KB series incubators and the fan speed is adjustable.
831 KF Coulometer-Metrohm
831 KF Coulometer includes titration cell and generator electrode with diaphragm for water content determination down to trace levels. This coulometer has several advantages due to its reliability, its backlit graphical display, the multi-language dialog and the simplicity of its operation. The clear display provides information regarding the relevant titration parameters and unambiguous indication of the progress of the titration in the form of a curve showing µg water over time. Recommended measuring range: 10 µg – 200 mg absolute water.
Raypa Digestion/Mineralization system
Digestion/Mineralization system by humidity is based on Kjeldahl method, the most accepted for the determination of nitrogen/protein in organic substances such as : nourishing products, feeds, fertilizers, vegetable grain, beer, meat, sauces, water, petroleum, etc. This compact system takes big advantage to the traditional method such as: simultaneous digestion from 6 to 20 samples; quick and safe manipulation of samples; reduction of the digestion time; reduction of chemical products, energy and space in the laboratory; efficient fume removal systems.
Eco Chemie Autolab Rotating Disk Electrode
The Eco Chemie Autolab Rotating Disk Electrode (RDE) is designed for electrochemical analysis. Featuring a sleek motor design and sealed, low-noise contacts, the RDE substantially minimizes the noise interference associated with rotating disk studies. Autolab RDE maintains speeds from 100 to 10,000 rpm, and it can be controlled manually or from a remote analog system, using different types of electrodes (from 3 to 5 mm diameter disks in Pt, Ag, Au and glassy carbon) to address a wide range of applications.
N. Radu, A. A. Chirvase, N. Babeanu, O. Popa, P. Cornea, L. Pirvu, M. Bostan, A. Ciric, E. Mathe, E. Radu, M. Doni, M. Constantin, I. Raut, A.-M. Gurban, M. Begea
Study Regarding the Potential Use of a Spent Microbial Biomass in Fertilizer Manufacturing
Agronomy Vol 10(2), 299, February 2020;10.3390/agronomy10020299
M. Caramihai,N. Radu,M. Bostan,, G. Ion, A. A. Chirvase, M. Constantin,M. Doni, N. Babeanu
Fuzzy Predictions Regarding In Vitro Behavior of Cells Line Under Cytostatic Treatment
IEEEXplore,January 2020, p 1-5.Electronic ISSN: 2575-5145, ISSN: 2575-5137.I.F. 4.463/ 10.1109/EHB47216.2019.8970088
Şesan, T. E., Oancea, A. O., Ştefan, L. M., Mănoiu, V. S., Ghiurea, M., Răut, I., Constantinescu-Aruxandei, D., Toma, A., Savin, S., Bira, A. F., Pomohaci, C. M., & Oancea, F.
Effects of Foliar Treatment with a Trichoderma Plant Biostimulant Consortium on Passiflora caerulea L. Yield and Quality
Microorganisms 8(1), 123; January 2020; 10.3390/microorganisms8010123
M Calin, I Raut, ML Arsene, M Doni, AM Gurban, L Jecu
Practical exemples regarding the valorization of cultural heritage through archaeometry studies using nuclear techniques and other scientific methods
Chapter 4. Isolation and identification of microorganisms involved in the biodeterioration of cultural heritage artifacts
I Raut, M Calin, ML Arsene, M Doni, AM Gurban, L Jecu
Valorization of cultural heritage through archaeometry studies using nuclear techniques and other scientific methods. General considerations and short literature survey
Chapter 5. Microbial deterioration of textile and wood artifacts
Gurban A.-M., Epure P., Raut I., Calin M., Vasilescu G., Jecu L., Arsene ML., Doni M.
Innovative sensing platforms for toxic compounds detection
15th Edition of the International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, October 30 – Nov. 01, 2019, Bucharest, Romania
Gurban A.-M., Epure P., Raut I., Calin M., Vasilescu G., Jecu L., Arsene M.L., Doni M.
Detection of aflatoxin M1 using a dual immunosensing platform
15th Edition of the International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, October 30 – Nov. 01, 2019, Bucharest, Romania