National Institute for Research & Development in Chemistry and Petrochemistry
ICECHIM
G6: Biotechnology & Bioanalysis
The 6th Research Group carries out research activities in the field of Biotechnology & Bioanalysis, the research directions being: 1. Biotechnologies for environmental protection and remediation - Biodegradation of waste and pollutants - Biotechnologies to remedy contamination 2. Agri-food and industrial biotechnologies for obtaining biologically active products - Microbial technologies for obtaining substances with antimicrobial properties - Pharmaceutical biotechnologies - Biotechnologies for obtaining products with uses in the light chemical and food industry (new sources of enzymes) 3. Bioanalysis methods for quality control of products and biotechnological processes in the field of health, environment and agri-food - Advanced methods and techniques of analysis, control and quality assurance of biotechnological substances and products - Development of sensors, biosensors and immunosensors for the detection of toxic compounds, pollutants and metabolites in the environment and agri-food products. Within the 6th reasearch group, there is a Collection of Microorganisms that contains fungi, yeasts and bacteria, which allows and supports the biotechnological approach of our research activities. Microorganisms have the ability to adapt to a variety of environmental conditions, their versatility making them useful for various biotechnological applications, such as removing pollutants from the environment, bioremediation or transformation into harmless compounds such as carbon dioxide and water. Enriching the collection of microorganisms by isolating them from various sources and maintaining them are of permanent concerns of microbiologists. The 6th research group is a multidisciplinary team, consisting in 2 chemists, 2 biochemists, 2 biologists, 1 engineer and 1 technician (3 CSI, 2 CSII, 2 CSIII; 6 doctors in science). The specialists our group actively participate in: - Evaluation of scientific research projects carried out at national (UEFISCDI) and European level (INTAS experts) - Review activity as references of specialized magazines from Romania and abroad - Roum. Biotechnol. Letters, Biosensors & Bioelectronics (Elsevier), Food Biotechnology, Sensors, Toxins, Biomachine, Biosensors, Bioelectrochemistry, Biomedical and Environmental Science, etc. - Supporting the activity of the Romanian Society of Bioengineering and Biotechnology (SRBB) (www.srbb.ro), an organization affiliated since 1993 to the European Biotechnology Federation - Didactic activities carried out in higher education institutions (University of Bucharest, Polytechnic University of Bucharest, etc.). Through Capacity project 49/CP/I/2007, the laboratories of the Biotechnology & Bioanalysis team were arranged according to GLP norms as follows: - Microbiology Laboratory (respecting the Rules of Good Laboratory Practice-RBPL regarding the flow of personnel, materials and samples) - Laboratory for (BIO) Sensors and optical methods of bioanalysis - Laboratory for Separation Methods
Senior Scientist 1st degree, Leader of Biotechnology & Bioanalysis Research Group
Dr. chem.
Extension: 144
Senior Scientist 3rd degree, Deputy Leader of Biotechnology & Bioanalysis Research Group
Dr. chem.
Extension: 144
Senior Scientist 1st degree, General Manager of INCDCP-ICECHIM
Dr. biochem.
Extension: 102
FINNIGAN Surveyor Plus HPLC, Thermo Scientific, USA
The Thermo Scientific Surveyor Plus HPLC is a robust modular system with a suite of integrated features to increase application flexibility and efficiency, being specifically optimized for the most demanding HPLC applications and allowing full customization for our laboratory's applications. The versatility of the modular design, featuring custom quaternary pumping solutions, provides flexibility for a wide variety of applications, from routine compound analysis to more complex matrices, offering short cycle times, high sample capacity and low flow rate performance.
Thermo Scientific NanoDrop-OneC, UV-Vis Spectrophotometer
The NanoDrop OneC is a Stand-Alone instrument with local control and Built-in touch screen, for improved measurement accuracy and contaminant identification. Using NanoDrop OneC spectrophotometer it is possible to quantify and qualify DNA, RNA, and protein samples by using only 1-2 µL, in seconds. The full-spectral data is obtained before decide to use samples in downstream applications. With contaminant analysis, sample information alerts and on-demand technical support, the NanoDrop One is the ideal UV-Vis spectrophotometer for life science research.
Thermo Spectronic BioMate UV-Vis spectrophotometer
Thermo Spectronic BioMate Double Beam UV-Vis Spectrophotometer with Brand quartz Cuvette, with soft for enzymatic and microbial kinetics for rapid, reliable nucleic acid and protein analysis, represents a grating-based spectrophotometer that can also be used as a general purpose instrument to measure other life science applications such as kinetics or multi wavelength analysis.
Sunrise Absorbance microplate reader - Tecan Life Science
Tecan’s Sunrise absorbance microplate reader is an ideal solution for a range of applications, such as ELISAs, enzyme kinetics, cell viability studies, veterinary assays and many more, offering all the functionality needed, including advanced 12-channel optics for fast, multichannel absorbance reading of ELISAs, a temperature control function for enzyme kinetic assays, and a tunable wavelength function for wavelength scanning. It supports a broad wavelength range – from 340 to 750 nm – and can measure an entire 96-well plate in less than six seconds, ensuring it is ready to meet the needs of routine diagnostic and research labs.
INNOVATIVE FULLERENOL - HYDROGELS BASED NANOMATERIALS FOR HEALTH DIAGNOSTIC AND CARE APPLICATIONS - FULSENS-GEL
The main objective of the project consists in the development of an innovative nanomaterial, based on combination of the elastic, flexible and resistant hydrogels with functional nanomaterials based on fullerenols
MANUFACTURING OF A PORTABLE SYSTEM FOR NITRITE MONITORING IN SOIL BASED ON AN INNOVATIVE SENSOR (NITRISENS)
NITRISENS aims to develop a method for manufacturing of a portable system for nitrite monitoring in soil based on an innovative electrochemical sensor inserted in a 3D printed soil solution extractor
Innovative MXene-peptides for electrochemical miniaturized platforms dedicated to tumor biomarker detection
The goal of MXEPEPMIN is to develop miniaturized biosensors based on innovative MXene-peptide nanomaterials for the detection of lung cancer biomarkers, CEA and EGFR.
Proiect 33/2017 MANUNET „Noi sisteme analitice pentru evaluarea prezentei toxinelor in industria lactatelor” (acronim TOX-HAZ-ASSESS)
Proiect 33/2017 MANUNET „Noi sisteme analitice pentru evaluarea prezentei toxinelor in industria lactatelor” (acronim TOX-HAZ-ASSESS)
ENHANCING FOOD SAFETY THROUGH DRUG RESIDUES MONITORING IN FOOD CHAIN USING INNOVATIVE BIOSENSITIVE PORTABLE TOOLS PN-IV-P7-7.1-PED-2024-1277
The main goal of the project is to develop miniaturized, cost-effective and robust biosensing instruments based on an innovative MXene-based nanomaterial customized with specific bioreceptors – an emerging class of 2D- conductive materials with super
BIOGENIC AMINES DETECTION IN FOOD BASED ON AN INNOVATIVE OPTO-ELECTROSENSITIVE PLATFORM
Scopul principal al proiectului îl constituie dezvoltarea unor platforme opto-electrosensibile, miniaturizate, rentabile și robuste bazate pe un nanomaterial inovator pentru determinarea specifică și sensibilă a aminelor biogene (BAs) din alimente
BioConstrMater
Biotechnological strategies for innovative construction materials incorporating bacterial bioproduct
Cristina Firincă, Mariana Constantin, Iuliana Răut, Lucian-Gabriel Zamfir, Maria-Luiza Jecu, Mihaela Doni and Ana-Maria Gurban
Fungal factories for feeding the future: mycoproteins as a sustainable protein source at the interface of food, health, and biotechnology innovation
Sustainable Food Technology
001742636800001
Cristina Firincă, Lucian-Gabriel Zamfir, Mariana Constantin, Iuliana Răut, Maria-Luiza Jecu, Mihaela Doni, Ana-Maria Gurban, and Tatiana Eugenia Sesan
Innovative Approaches and Evolving Strategies in Heavy Metal Bioremediation: Current Limitations and Future Opportunities
Journal of Xenobiotics
https://doi.org/10.3390/jox15030063
Lucian-Gabriel Zamfir, Iuliana Răut, Mariana Constantin, Nicoleta Olguța Corneli, Cristina Firincă, Maria-Luiza Jecu, Petru Epure, Cristina Lavinia Nistor, Mihaela Doni, Ana-Maria Gurban
Assessment of biogenic amines produced by microorganisms as food spoilage indicators by sensitive detection using portable opto-electrochemical tools based on biosensors
Food Control
001423049000001
Răut, I.; Constantin, M.; Suică-Bunghez, R.; Firincă, C.; Alexandrescu, E.; Gîfu, I.C.; Doni, M.; Zamfir, L. G.; Gurban, A.M.; Jecu, L.
Extracellular Biosynthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Synthesized by Filamentous Fungi
J. Fungi
https://doi.org/10.3390/jof10110798
C Firincă, LG Zamfir, M Constantin, I Răut, L Capră, Diana Popa, ML Jinga, AM Baroi, RC Fierăscu, NO Corneli, C Postolache, M Doni, AM Gurban, Luiza Jecu, TE Șesan,
Microbial Removal of Heavy Metals from Contaminated Environments Using Metal-Resistant Indigenous Strains,
J. Xenobiot
https://doi.org/10.3390/jox14010004
Stefaniu A.; Neagu, G.; Albulescu A.; Radu, N.; Pirvu, L.C
In silico comparison of quantum and bioactivity parameters of a series of natural diphenyl acetone analogues; in vitro Caco-2 studies on three main chalcone derivates
Symmetry, 3222087, 2024
https://doi.org/10.3390/sym16101383
Idu, A.-A.; Albu Kaya, M.G.; Rău, I.; Radu, N.; Dinu-Pîrvu, C.-E.; Ghica, M.V.
Novel Collagen Membrane Formulations with Irinotecan or Minocycline for Potential Application in Brain Cancer
Materials 2024, 17, 3510.
https://doi.org/10.3390/ma17143510
Viviana Roman, Mirela Mihaila, Nicoleta Radu, Stefania Marineata, Carmen Cristina Diaconu and Marinela Bostan
Cell Culture Model Evolution and Its Impact on Improving Therapy Efficiency in Lung Cancer
Cancers 2023, 15, 4996
https://doi.org/10.3390/ cancers15204996
Mariana Constantin, Iuliana Răut, Raluca Suica-Bunghez , Cristina Firinca, Nicoleta Radu , Ana-Maria Gurban , Silviu Preda , Elvira Alexandrescu , Mihaela Doni and Luiza Jecu
Ganoderma lucidum-Mediated Green Synthesis of Silver Nanoparticles with Antimicrobial Activity
Materials 2023, 16, 4261
https://doi.org/ 10.3390/ma16124261
Gabriela Petcu, Florica Papa, Elena Maria Anghel , Irina Atkinson, Silviu Preda , Simona Somacescu, Daniela C. Culita , Adriana Baran, Elena Madalina Ciobanu, Luiza Maria Jecu , Mariana Constantin and Viorica Parvulescu
Effects of Aluminosilicate Gel Treatment and TiO2 Loading on Photocatalytic Properties of Au–TiO2/Zeolite Y
Gels 2023, 9, 503
Y. Gels 2023, 9, 503. https://doi.org/10.3390/ gels9060503 Academic Edi
Calin M; Raut I.; Gurban A.-M.; Doni M.; Radu N.; Jecu L.
Archaeometrical study of selected objectives from Golesti Museum of Viticulture and Pomiculture
Chapter5: Microbial contamination of wooden cultural heritage artifacts of selected objectives from Golesti Museum
ISBN978-619-91466-1-3
Radu N.; Babeanu N.; Cornea P.; Calin M.; Jecu L.; Gurban A.M; Doni, M;
Archaeometrical study of selected objectives from Golesti Museum of Viticulture and Pomiculture
Chapter 4:Paintings characteristics from inside of wooden church achieved in the period 1750-1850
ISBN 978-619-91466-1-3
M Calin, I Raut, ML Arsene, M Doni, AM Gurban, L Jecu
Practical exemples regarding the valorization of cultural heritage through archaeometry studies using nuclear techniques and other scientific methods
Chapter 4. Isolation and identification of microorganisms involved in the biodeterioration of cultural heritage artifacts
ISBN: 978-973-0-27114-0
I Raut, M Calin, ML Arsene, M Doni, AM Gurban, L Jecu
Valorization of cultural heritage through archaeometry studies using nuclear techniques and other scientific methods. General considerations and short literature survey
Chapter 5. Microbial deterioration of textile and wood artifacts
ISBN: 978-973-0-27114-0
F. Oancea, I. Raut, T.E .Sesan, M. Doni, M. Popescu, V. Zamfiropol-Cristea, L. Jecu
Trichoderma spp. - applications in agriculture and horticulture
Chapter 8 - Formulation of the Multifunctional Trichoderma Strains
ISBN 978-606-16-0900-0
F., Oancea, I. Raut, T.E. Sesan, M. Doni, M. Popescu, V. Zamfiropol-Cristea, L. Jecu
Trichoderma spp. - applications in agriculture and horticulture
Chapter 7. Trichoderma Strains as Plant Biostimulants in High Residues Farming Systems
ISBN 978-606-16-0900-0
Răut I., Şesan T.E., Arsene M.L.*, Badea-Doni M., Jecu L., Călin M., Vasilescu G., Oancea F.
Trichoderma spp. - applications in agriculture and horticulture
Chapter 5. Volatile and non-volatile metabolites of Trichoderma with biocontrol implications
ISBN 978-606-16-0900-0
Arsene M.L.*, Jecu L., Badea-Doni M., Raut I., Oancea F., Calin M
Trichoderma spp. - applications in agriculture and horticulture
Chapter 4. Bioactivity and biological role of secondary metabolites produced by Trichoderma spp
ISBN 978-606-16-0900-0
Arsene M.L., Badea-Doni M., Jecu L*, Răut I., Călin M
Trichoderma spp. - applications in agriculture and horticulture
Chapter 3. Chemical structure of secondary metabolites
ISBN 978-606-16-0900-0
A.F. Danet, M. Badea – Doni
Flow Injection Analysis of Food Additives
Chapter 32: Determination of Lipid Oxidation by Chemiluminescence Reagents - pp. 623-638, CRC Press, Francis & Taylor Group, Editors: Claudia Ruiz-Capillas, Leo M. L. Nollet (2015)
ISBN 9781482218190
Cristina Firincă, Lucian-Gabriel Zamfir, Iuliana Răut, Mariana Constantin, Radu Claudiu Fierăscu, Luiza Capră, Mihaela Doni, Luiza Jecu, Ana-Maria Gurban, Tatiana Șesan
Synergistic Fungal–Plant Systems Integrated With Electrochemical Monitoring For Sustainable Heavy Metal Remediation In Soil
”The International Symposium Priorities of chemistry for a sustainable development″ - PRIOCHEM XXth, 15-17 octombrie 2025, Bucuresti
Cristina Firincă, Lucian-Gabriel Zamfir, Mariana Constantin, Iuliana Răut, Luiza Jecu, Mihaela Doni, Radu-Claudiu Fierăscu, Ana-Maria Gurban
Integrated Bioremediation And Monitoring Of Chromium-Contaminated Soils Using Fungal Consortium And Electrochemical Sensors
8th International Conference on Emerging Technologies in Materials Engineering EmergeMAT, 6-7 octombrie 2025, București, România
C. Firincă, I. Răut., M.-L. Jinga, L. Capră, R. Constantinescu, A.-M. Gurban, L.-G. Zamfir M. Doni, M.L. Jecu, M. Constantin, C. Postolache, T. E. Șesan
Bioremediation strategy for chromium contaminated soils
Next-Chem Tehnologii Inovatoare Trans-Sectoriale – workshop exploratoriu, ediția a V-a, 22-23 May 2023, Bucharest, Romania
R. Ianchiş, E. Alexandrescu, I.C. Gîfu, I.C. Mihăescu, C.L. Nistor, C. Petcu, L.-G. Zamfir, M. Doni, A.-M. Gurban
Novel biopolymer-polyaniline composites designed for sensing applications
4th International Conference on Emerging Technologies in Materials Engineering – EmergeMAT, 4–5 November 2021, Bucharest, Romania
L.-G. Zamfir, P. Epure, C. Mitrea, M. Constantin, I. Raut, M.-L. Jecu, M. Doni, A.-M. Gurban
A comparative study of different nanomaterials used in the development of sensitive electrochemical sensors for nitrite determination and monitoring in soil
6th edition of International Conference on Analytical and Nanoanalytical Methods for Biomedical and Environmental Sciences "IC-ANMBES 2022", June 8–10 2022, Brașov, Romania
Marilena Gabriela Zaharie, Mihai Caramihai, Montserrat Adroguer, Paqui Blánquez, Tom Kuppens and Nicoleta Radu
Virtual Reality and Mixed Reality Education: A Solution for Today’s Education in a Circular Bioeconomy
16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020.
Mariana Călin, Iuliana Răut, Ana-Maria Gurban, Mihaela Doni, Nicoleta Radu, Gelu Vasilescu and Luiza Jecu
Assessment of Biological Contamination from Wooden Artifacts of Golesti Museum
16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020
Iuliana RĂUT, Mariana CĂLIN, Ana-Maria GURBAN, Luiza CAPRĂ, Mihaela DONI, Nicoleta RADU, Gelu VASILESCU and Luiza JECU
A Biotechnological Process for Valorizing Poultry Feathers through Keratinolytic Cladosporium Isolates
16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020.
Naomi Tritean, Mariana Călin, Iuliana Răut, Ana-Maria Gălan, Alexandru Vlaicu, Diana Constantinescu-Aruxandei and Florin Oancea
Extraction of Proteins from Microalgae Using Lytic Enzymes Produced by Trichoderma Strains
16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020.
Nicoleta Radu, Narcisa Babeanu, Petruta Cornea, Mariana Calin , Luiza Jecu, Iuliana Raut, Ana Maria Gurban and Mihaela Doni
Painting Degradation from Inside Wooden Churches Achieved in the Period 1750–1850
16th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 28–30 October 2020.
Zamfir L.-G., Gurban A.-M., Doni M., Jinga M.- L., Răut I., Constantin M., Jecu M.L.
Procedeu de realizare a unor biosenzori electrochimici bazaţi pe nanomateriale pentru determinarea de amine biogene
RO138657
Firincă C., Zamfir L.-G., Gurban A.-M., Doni M., Constantin M., I. Răut, R.-I. Matei, M.L. Jecu
Procedeu de bioremediere in-situ a solurilor poluate cu crom hexavalent, utilizând consorțiu pe bază de Trichoderma și Clonostachys
A00554/2025
Constantin M., Răut I., Şuică-Bunghez I.-R., Gurban A.-M., Firincă C., Zamfir L.-G., Vasilescu G., Radu N., Jecu M.L.
Biomaterials with silver nanoparticles and Ganoderma Lucidum metabolites and preparation process
Nr. dosar: RO202300123A cerere de brevet RO138396
Gurban A.-M.; Zamfir L.-G.; Doni M.; Răut I.; Constantin M.; Firincă C.; Jecu M.-L.
Procedeu de realizare a unei platforme multi-senzitivă bazată pe nanomateriale inovative pentru monitorizarea unor biomarkeri de importanță clinică
A 2024-00687
Radu N., Constantin M., Răut I., Vasilescu-Panea G., Gurban A.-M., Doni M., Jecu M.-L.
Culture medium composition for sporulation
A2021-00663, RO137459 (A2) publicat în BOPI 5 / 30.05.2023
Gurban A.-M., Doni M., Zamfir L.-G., Jecu M.-L., Răut I., Constantin M.
Process for making a miniaturized electrochemical sensor for determining nitrites in soil
A2022-00743, RO137419 (A0) publicat în BOPI 4 / 28.04.2023
Constantin M., Răut I., Bunghez Șuică R., Gurban Ana-Maria, Firincă C., Zamfir L.-G., Vasilescu G., Radu N., Jecu M.-L.
Biomateriale cu nanoparticule de argint şi metaboliţi de Ganoderma lucidum şi procedeu de obţinere a acestora
A2023-00123 / 14.03.2023
Sandu, T, Sarbu A, Jecu L, Iordache T, Gavrilsa AM, Apostol L, Radu AL, Zaharia AM, Arsene ML
Perle polimerice cu continut de particule magnetice si procedeu de obtinere a acestora
Brevet RO 134595/2023
Cornea PC, Oancea F, Voaideş CM, Răut I, Boiu Sicuia OA, Constantinescu Aruxandei D, Doni M, Jecu L
Consorţiu microbian multifuncţional pentru solubilizarea fitosiliciului şi procedeu pentru utilizarea acestuia
Brevet RO133468B1/2023.
Oancea F, Călin M, Constantinescu D, Răut I, Doni M, Arsene ML, Jecu L,
Compozitie de biostimulant pentru plante pe baza de lana, si procedeu de obtinere,
Brevet RO133240 B1/2021.
Doni M; Jecu L.; Gurban AM.; Radu N.; Constantin M; Raut I; Firinca C.; Zamfir L
CONFERENCE-AWARDS
R.M. Ion; N. Ion; L. Iancu; N. Radu
Silver Medal awarded to Patent request to EUROINVENT, 11th Edition, European Exhibition of Creativity and Innovation, May 18, 2019, Iasi, Romania.
R.M. Ion, N. Ion, L.Iancu, N. Radu
Gold Medal awarded to Patent request 17th edition of PROINVENT, March 20-22, 2019, Cluj Napoca, Romania
